This little experiment was inspired by Adrian (Adrian's Images) when he photographed Brian the Snail on a mirror. Following that we exchanged some ideas about types of mirror. I purchased a cheap A5 front surfaced acrylic 3mm thick mirror and finally got Round Tuit with an experiment. My subject was a miniature bullrush cut from my garden pond positioned so it would be in focus but the reflection would be well out of focus:
The 50D was fitted with 70-200mm lens plus Raynox DCR-150 macro lens. The camera was tethered to the MacBook so a clear large picture could be seen of what the camera was looking at. Four focus stacked shots were taken and processed with Zerene Stacker:
The orange background was from some cupboard doors seen reflected in the mirror. The only lighting was natural daylight / Sunlight coming through the kitchen window.